These software applications determine the properties of any oligo sequence entered, as well as facilitate the intelligent design of assay conditions, all at the click of a button. The suite is easy to use and makes it simple to add each optimized oligonucleotide to an ongoing order list ready for purchase.
Identifies oligonucleotide properties, including melting temperature, hairpins, dimers and mismatches
Identifies oligonucleotide properties, including melting temperature, hairpins, dimers and mismatches
Find the volume needed to dilute your oligo stock to a lower concentration
Find the volume needed to resuspend a dry oligo to a desired concentration
Selects qPCR primers and probe sequences for human, mouse, and rat gene expression assays based on up-to-date sequence data
Allows highly customized designs for PCR primers and qPCR assays
Selects custom qPCR assays for any species
Select dye combinations that are compatible with your qPCR instrument
Choose predesigned rhAmp SNP Assays or design assays for SNPs not currently included in the predesigned assay library
Selects guaranteed crRNAs targeting human, mouse, rat, zebrafish, or nematode genes
Designs custom crRNAs to sequences from any species
A complete solution for industry-leading HDR rates based on extensive wet bench testing and customer validation
Assesses on- and off-targeting potential of user-designed protospacer sequences
Flexible, cloud-based interrogation of CRISPR-mediated, double-strand break repair
Selects DsiRNAs and TriFECTa® RNAi Kits for your human, rat, or mouse targets
Generates DsiRNAs for any sequence from any species
Automates codon optimization of a gene to the species of your choice
Track your order (Genes & gene fragments only)
Guides you through choice of library prep method, configuration, indexing strategy, delivery format, and other options. Order directly online.
Please use the following format when citing SciTools:
PrimerQuest™ program, IDT, Coralville, Iowa, USA. Accessed 12 December, 2018. https://www.idtdna.com/SciTools.